PMS 2 · Genetics of microorganisms and microbial biotechnologies
Thursday .::. 27 September .::. 14:00-16:00

PMS.2 · O+P1    

Identification of the cellular target of the potential antitubercular drug BM212
S. Buroni, G. Manina, G. Riccardi, E. de Rossi
Dept Genetica e Microbiologia, Univ. Pavia (Pavia)

PMS.2 · O+P2    

Characterization of a sigF mutant in Mycobacterium smegmatis
R. Provvedi¹, D. Kocincova², V. Donà¹, G. Etienne³, D. Euphrasie², M. Daffé³, R. Manganelli¹, J.M. Reyrat^2
1Dept Biology, Univ. Padua (Padua); ²Univ Paris Descartes, Facultè de Medecine Renè Descartes, Inserm (Paris, France); ³Institut de Pharmacologie et de Biologie Structurale, Unitè Mixte de Recherche du Centre National de la Recherche Scientifique et de l’Univ Paul Sabatier, Dept Mecanismes Moleculaires des Infections Mycobacteriennes (Toulouse, France)

PMS.2 · O+P3    

Combating antibiotic resistance in S. pneumoniae infections: identification of neuraminidases binding peptides
J. Conti¹, G. Gioffrè¹, J. Athwal², A. Romeo¹, I. Pernice¹, P.W. Andrew³, M. Oggioni², F. Felici^1
1Dept Microbiological Genetic and Molecular Sciences, Univ. Messina (Messina); ²LAMMB, Dept of Molecular Biology, Univ. of Siena (Siena); ³Dept of Infection, Immunity and Inflammation, University of Leicester (Leicester, United Kingdom)

PMS.2 · O+P4    

Proteomic analysis of Amycolatopsis balhimycina to reveal primary metabolism proteins involved in balhimycin production
G. Gallo¹, R. Alduina¹, G. Renzone², L. Lo-Piccolo¹, C. Taurino¹, H. Echenique-Rivera¹, A. Scaloni², A.M. Puglia^1
1Dip. Biologia Cellulare e dello Sviluppo, Univ. Palermo (Palermo); ²Proteomics and Mass Spectrometry laboratory, ISPAAM, CNR (Naples)

PMS.2 · O+P5    

Cis-acting elements in pnp mRNA 5'-UTR and their role in autoregulation
T. Carzaniga, S. Zangrossi, G. Merlino, G. Deho', F. Briani
Dip. Scienze Biomolecolari e Biotecnologie, Univ. Milano (Milano)

PMS.2 · O+P6    

Secreted proteases reflect metabolic specialization in Dermatophytes
O. Jousson¹, M. Monod^2
1Centre for Integrative Biology, Univ. Trento (Trento); ²Dermatology Service, Univ. Hospital Lausanne (Lausanne, Switzerland)

PMS.2 · O+P7    

Constitutive expression of glycopeptide resistance in Actinoplanes teichomyceticus ATCC31121 is mediated by a defective vanS gene
F. Beltrametti², G.L. Marcone¹, L. Carrano¹, J.H. Hong³, M. Buttner³, F. Marinelli^1
1DBSM, Univ.Insubria (Varese); ²Actygea S.r.l., Gerenzano (Varese); ³Dept of Molecular Microbiology, John Innes Centre, Colney (Norwich, United Kingdom)

PMS.2 · O+P8    

pga gene expression and production of poly-N-acetyl-glucosamine (PNAG) in a biofilm-forming mutant of Escherichia coli
L. Tagliabue¹, E. Brombacher², C. Dorel³, P. Landini^1
1Dept Biomolecular Sciences and Biotechnology (Milan); ²Swiss Institute of Technology (Zurich, Switzerland); ³Unité de Microbiologie et Génétique, Institut National des Sciences Appliquées de Lyon (Lyon, France)

PMS.2 · P9    

Set-up of functional assays to determine structure-function relationship of the CsgD and AdrA biofilm determinants
D. Antoniani, L. Tagliabue, P. Landini
Dept Biomolecular Sciences and Biotechnology (Milan)

PMS.2 · P10    

Biofilm development in L. plantarum
C. Marino¹, L. Muscariello¹, I. Pommella¹, M. Palmieri¹, R. Marasco², M. de Stefano¹, M. Sacco^1
1Dip. Scienze Ambientali, Seconda Univ. Napoli, Caserta (Caserta); ²Dept di Scienze della Vita, Seconda Univ. di Napoli,Caserta (Caserta)

PMS.2 · P11    

Anti adhesion effect of biosurfactants against biofilm formation
F. Rivardo^1,2, M.G. Martinotti^1,2, R.J. Turner^3,4, H. Ceri^3,4
1Dept of Chemical, Food, Pharmaceutical and Pharmacological Science, Univ. Eastern Piedmont (Novara); ²Drug and Food Biotechnology Centre (DFBC) (Novara); ³Dept of Biological Sciences, Univ. of Calgary (Calgary, Canada); ⁴Biofilm Research Group (Calgary, Canada)

PMS.2 · P12    

Characteristics of biosurfactants produced by bacteria and whey waste as alternative source for their production
M.G. Martinotti¹, G. Allegrone¹, F. Sinigaglia², F. Rivardo^1
1DiSCAFF, Univ. Eastern Piedmont (Novara); ²Dept Medical Sciences, Univ. Eastern Piedmont (Novara)

PMS.2 · P13    

Effector cells from Paracentrotus lividus as source of new anti-staphylococci biofilm agents
D. Schillaci¹, V. Arizza², A. Di Silvestre^1
1Dept Pharmaceutical Chemistry and Technology, Univ. Palermo (Palermo); ²Dept Animal Biology, Univ. Palermo (Palermo)

PMS.2 · P14    

Molecular characterization of Stenotrophomonas maltophilia isolated from cystic fibrosis patients
M. Casalino¹, A. Iacobino¹, G. Prosseda², S. Cannavacciuolo³, E. Fiscarelli^4
1Dept Biology, Univ. Roma3 (Rome); ²Dept Cell and Developmental Biology, Univ. La Sapienza (Rome ); ³Dept Biomedical Sciences, Univ. G. D'Annunzio (Chieti ); ⁴Hospital Bambin Gesù (Rome)

PMS.2 · P15    

Virulence factors of Stenotrophomonas maltophilia, an emergent pathogen associated to cystic fibrosis
G. Prosseda¹, G. Di Bonaventura², V. Michelacci¹, M.L. Di Martino¹, F. Del Chierico², B. Colonna^1
1Dept Cell and Developmental Biology, Sapienza Univ. (Rome); ²Dip. Scienze Biomediche, Univ. G. D'Annunzio (Chieti)

PMS.2 · P16    

Combating antibiotic resistance in S. pneumoniae infections: identification of pneumolysin binding peptides
G. Gioffrè¹, A. Romeo¹, R. El-Rachkidy², C.L. Passo¹, M.R. Oggioni³, G. Teti⁴, P.W. Andrew², F. Felici^1
1Dip. Scienze Microbiologiche, Genetiche e Molecolari (Messina); ²Dept of Infection, Immunity and Inflammation, Univ. of Leicester, UK (Leicester, United Kingdom); ³LAMMB, Dept of Molecular Biology, Univ. of Siena (Siena); ⁴Dept of Pathology and Experimental Microbiology, Univ. of Messina (Messina)

PMS.2 · P17    

Influence of Pseudomonas aeruginosa on the growth rate and internalization of  Burkholderia cenocepacia
L. Pirone^1,2, S. Marinelli¹, C. Auriche¹, C. Dalmastri², A.M. Bevivino², F. Ascenzioni^1
1Dip. Biologia Cellulare e dello Sviluppo, Univ. "La Sapienza" (Rome); ²Dept of Biotechnologies, Agroindustry and Protection of Health, ENEA C.R. Casaccia (S. Maria di Galeria)

PMS.2 · P18    

Grafting of VH-CDR3 from anti-pneumolysin monoclonal antibodies into Phage Display vectors
A. Romeo¹, G. Gioffrè¹, I. Pernice¹, C.L. Passo¹, M.R. Oggioni³, G. Teti⁴, P.W. Andrew², F. Felici^1
1Dept Microbiological Genetic and Molecular Sciences, Univ. Messina (Messina); ²Dept of Infection, Immunity and Inflammation, Univ. of Leicester, UK (Leicester, United Kingdom); ³LAMMB, Dept of Molecular Biology, Univ. of Siena (Siena); ⁴Dept of Pathology and Experimental Microbiology, Univ. of Messina (Messina)

PMS.2 · P19    

Peptide Mimics of the Group B Menigococcal Capsule Induce Bactericidal and Protective Antibodies after Immunization
C. Lo Passo¹, A. Romeo¹, I. Pernice¹, A. Midiri², G. Mancuso², R. Galbo², F. Felici¹, C. Beninati^2
1Dip. di Scienze Microbiologiche, Genetiche e Molecolari, Univ. Messina (Messina); ²Dept of Pathology and Experimental Microbiology, Univ. of Messina (Messina)

PMS.2 · P20    

PE is a functional domain responsible for protein translocation and localization on mycobacterial cell wall
A. Cascioferro¹, G. Delogu², M. Colone³, M. Sali², A. Stringaro³, G. Arancia³, G. Fadda², G. Palù¹, R. Manganelli^1
1Dept of Histology, Microbiology and Medical Biotechnologies (Padua); ²Institute of Microbiology, Catholic Univ. of Sacred Heart, (Rome); ³Dept of Technology and Health, National Institute of Health (Rome)

PMS.2 · P21    

Alternative translational start sites of SigE, an ECF sigma factor of M. tuberculosis
V. Donà, E. Dainese, A. de Iulis, G. Palù, R. Manganelli, R. Provvedi
Dept Istology, Microbiology and Medical Biotec., Univ. Padua (Padua)

PMS.2 · P22    

Development of a reverse inducible system for conditional expression in mycobacteria based on Pip-TetR repressors
F. Boldrin, S. Casonato, G. Palù, R. Manganelli
Dept of Histology, Microbiology and Medical Biotechnologies, Univ. Padua (Padua)

PMS.2 · P23    

Azole drug resistance in Mycobacterium tuberculosis
R. Provvedi², M.R. Pasca¹, A. Milano¹, A.P. Lucarelli¹, G. Manina¹, R. Manganelli³, G. Riccardi^1
1Dip. di Genetica e Microbiologia, Univ. of Pavia (Pavia); ²Dip. di Biologia, Univ. di Padova (Padova); ³Dip. di Istologia, Microbiologia e Biotecnologie Mediche, Univ. di Padova (Padova)

PMS.2 · P24    

Second generation PBOs show enantioselective binding to the catalytic complex of HIV-1 RT wt and drug resistant mutants
A. Amoroso¹, A. Samuele¹, G. Campiani², A. Karytinos¹, M. Facchini¹, S. Zanoli¹, G. Maga^1
1Inst Molecular Genetics IGM-CNR, Univ. Pavia (Pavia); ²Dept of Pharmaceutical Chemical Technology and European Research Center for Drug Discovery and Development, Univ. of Siena (Siena)

PMS.2 · P25    

Vancomycin-resistance transferability from VanA enterococci to Staphylococcus aureus
M. Bondi, I. Anacarso, S. de Niederhausern, E. Guerrieri, C. Sabia, R. Iseppi, G. Manicardi, P. Messi
Dept Biomedical Sciences, Univ. Modena and Reggio E. (Modena)

PMS.2 · P26    

Aromatic aminoacids and antibiotic production in Streptomyces coelicolor
S. Marineo¹, A. Giardina¹, E. Laing², G. Bucca², C.P. Smith², M.G. Fanale¹, S. Mandalà¹, A.M. Puglia^1
1Dept Cellular and Developmental Biology, Univ. Palermo (Palermo); ²School of Biomedical and Molecular Sciences, Univ. Guildford (Guildford, United Kingdom)

PMS.2 · P27    

PNPase-dependent stabilization of Escherichia coli mRNA in S1 ribosomal protein overexpressing cells
F. Briani, T. Carzaniga, S. Curti, F. Rossi, G. Dehò
Dip. di Scienze biomolecolari e Biotecnologie, Univ. Milano (Milan)

PMS.2 · P28    

The StyS histidine kinase, a key regulatory element of the Pseudomonas fluorescens ST styrene catabolic pathway
L. Leoni, G. Rampioni, C. Micolonghi, F. Massai, P. Ascenzi, E. Zennaro
Dept Biology, Univ. Roma Tre (Rome)

PMS.2 · P29    

RsaL, a quorum sensing homeostatic effector controlling virulence and biofilm formation in Pseudomonas aeruginosa
G. Rampioni¹, M. Schuster², E.P. Greenberg³, I. Bertani⁴, M. Grasso¹, V. Venturi⁴, E. Zennaro¹, L. Leoni^1
1Dept Biology, Univ. Roma Tre (Rome); ²Dept Microbiology, Univ. Oregon State (Corvallis, OR, USA); ³Dept Microbiology, Univ. Washington (Seattle, WA, USA); ⁴ICGEB (Trieste)

PMS.2 · P30    

Repression at a bacterial enhancer: interplay between the LytTR response regulator PprA and the σ⁵⁴-dependent activator XylR
E. Vitale, A. Milani, F. Renzi, E. Galli, E. Rescalli, G. Bertoni
Dept Biomolecular Sciences and Biotechnology, Univ. Milan (Milan)

PMS.2 · P31    

Cell engineering of Pseudoalteromonas haloplanktis TAC125: construction of a mutant strain with reduced exo-proteolytic activity
E. Parrilli, G. Marino, M.L. Tutino
Dip. Chimica Organica e Biochimica, Univ. Napoli "Federico II" (Naples)

PMS.2 · P32    

towards a truffles genetics
S. Federico¹, A. Brenna¹, P. Filetici³, P. Ballario^1
1Dip. Genetica e Biologia Molecolare, Univ. (Rome); ²Dip. GBM La Sapienza (Rome)

PMS.2 · P33    

Exposing yeasts to an acid stress: a chemostat study
L. Brambilla, D. Porro
Dept Biotechnol. & Biosciences, Univ. Milano-Bicocca (Milan)

PMS.2 · P34    

Comparative Functional Genomics of glucose repression in laboratory and wine yeasts
E. Marchi¹, T. Lodi², P. Goffrini², L. Rizzetto³, C. de Filippo³, D. Cavalieri^3
1Dept pf Biochemical Sciences, Univ. Firenze (Firenze); ²Dept Genetics, Biologia dei Microrganismi, Antropologia, Evoluzione, Univ. Parma (Parma); ³Dip. Farmacologia Preclinica e Clinica, Univ. Firenze (Firenze)

PMS.2 · P35    

New PCR identification of Candida dubliniensis by using a single primer pair
O. Romeo, G. Criseo
Dept microbiological, Genetic and Molecular Sciences, Univ. Messina (Messina)

PMS.2 · P36    

Development of a sensitive and specific assay combining multiplex-PCR and T-RFLP for the detection of Pseudomonas spp.
C. Cecilia, M. Ruzzi
Dept Agrobiology and Agrochemistry, Univ. La Tuscia, Viterbo (Viterbo)

PMS.2 · P37    

Recombinant protein glycosylation, stress responses and mitochondria: the calcium connection
E. Zanni¹, D. Uccelletti¹, F. Farina², P. Mancini³, C. Palleschi^1
1Dept Developmental and Cell Biology, Univ. La Sapienza (Rome); ²Institut de genetique et microbiologie, Univ. Paris-sud (Paris, France); ³Dept Experimental Medicine and Patology, Univ. La Sapienza (Roma)

PMS.2 · P38    

Barriers to horizontal gene transfer: the case of bacterial sister species Sinorhizobium meliloti and S. medicae
L. Ferri, A. Gori, S. Petreni, A. Mengoni, E.G. Biondi, M. Bazzicalupo
Dept Animal Biology and Genetics (Florence)

PMS.2 · P39    

Use of the cell wall protein Pir4 as a fusion partner for the expression of heterologous proteins in S. cerevisiae
F. Romano¹, E. de Alteriis¹, I. Andrès², J. Zueco², M. Bianchi³, L. Paciello⁴, V. Romano⁴, P. Parascandola^4
1Dip. Biologia Strutturale e Funzionale, Univ. Napoli "Federico II" (Naples); ²Unidad Microbiologia, Facultad de Farmacia, Univ. de Valencia (Valencia, Spain); ³Dip. Biologia Cellulare e dello Sviluppo, Univ. "La Sapienza" (Rome); ⁴Dip. Ingegneria Chimica e Alimentare, Univ. di Salerno (Salerno)

PMS.2 · P40    

Regulation of the KlPDC1 gene by hypoxia and autoregulation: basic research and applications
C. Micolonghi¹, M. Wésolowski-Louvel², M. Lemaire², L. Brambilla³, D. Porro³, G. Damato¹, M.M. Bianchi^1
1Dept of Cell and Developmental Biology, Univ. of Rome 'La Sapienza' (Rome); ²Génétique des Levures, Univ. Lyon 1 (Lyon, France); ³Dept of Biotechnology and Biosciences, Univ. of Milan-Bicocca (Milan)

PMS.2 · P41    

Yeast equivalents of disease-associated human Polg mutations show deleterious cooperative effects in vivo
E. Baruffini¹, I. Ferrero¹, F. Foury^2
1Dip. Genetica, Biologia dei Microrganismi, Antropologia, Evoluzione, Univ. Parma (Parma); ²Unitè FYSA, Univ. Catholique de Louvain (Louvain-la-Neuve, Belgium)

PMS.2 · P42    

Characterization of the Saccharomyces cerevisiae SYM1 gene, ortholog of the human disease gene MPV17
C. Dallabona, R.M. Marsano, C. Donnini
Dip. Genetica, Biologia dei Microrganismi, Antropologia, Evoluzione, Univ. Parma (Parma)

PMS.2 · P43    

Biotransformation of monoterpenes:asymmetric reduction of (4S)-(+)-carvone by yeast whole-cells
M. Goretti¹, C. Ponzoni², M.R. Cramarossa², E. Branda¹, P. Buzzini¹, B. Turchetti¹, L. Forti^2
1Dip. di Biologia Applicata, Univ. Perugia (Perugia); ²Dip. di Chimica, Univ. Modena and Reggio Emilia (Modena)

PMS.2 · P44    

Biovanillin production from ferulic acid by employing Pseudomonas fluorescens BF13-1p
A. Negroni¹, L. Sciubba¹, D.D. Gioia¹, M. Ruzzi², F. Fava^1
1DICASM, Univ. Bologna (Bologna); ²DABAC, Univ. La Tuscia (Viterbo)

PMS.2 · P45    

Biocatalyst expressing cis-naphthalene dihydrodiol dehydrogenase catalyzes alcohol and 1,2-diol dehydrogenase reactions
P.D. Gennaro¹, G. Bestetti¹, P. Mattavelli², S. Bernasconi², F. Orsini², G. Sello^2
1Dip. Scienze Ambiente e Territorio, Univ. Milano-Bicocca (Milan); ²Univ. Milano (Milano)

PMS.2 · P46    

Characterization of novel N,N-DABOs as effective tight binding non-nucleoside inhibitors of HIV-1 RT
M. Facchini¹, A. Samuele¹, L. Ferrigno¹, A. Karytinos¹, D. Rotili², A. Mai², G. Maga^1
1Inst Molecular Genetics IGM-CNR, Univ. Pavia (Pavia); ²Inst Pasteur, Dept Pharmaceutical Research, Univ. La Sapienza (Rome)

PMS.2 · P47    

Naphthalene and o-xylene degradation in Rhodococcus opacus R7:identification of genes involved in the catabolic pathways
P. Di Gennaro¹, P. Terreni¹, F. de Ferra², F. Rodriguez², G. Bestetti^1
1Dept Environmental Sciences, Univ. Milano-Bicocca (Milan); ²EniTecnologie (S.Donato Milanese)

PMS.2 · P48    

Novel HIV-1 NNRTIs with reduced loss of potency against common drug-resistant mutations
S. Zanoli¹, K.F. Adebambo², R. Cancio¹, M.G. Thomas², N.M. Howarth², G. Maga^1
1Istituto di Genetica molecolare del CNR di Pavia (Pavia); ²Chemistry, School of Engineering & Physical Sciences, William H.Perkin Building, Heriot-Watt University (Edimburg, United Kingdom)

PMS.2 · P49    

Expression, characterization of human DDX3 protein and construction of mutants
A. Garbelli, R. Franca, A. Belfiore, G. Maga
Inst Molecular Genetics IGM-CNR, Univ. Pavia (Pavia)

PMS.2 · P50    

Adenovirus-induced activation of the human prion
P. Caruso¹, R.B. ¹, Y. Martina², S. Piersanti^1,3, C. Remoli^1,3, I. Saggio^1,3,4
1Dept of Genetics and Molecular Biology, Univ. of Rome, "La Sapienza" (Rome); ²adaltis SPA (Aprilia); ³Fondazione Parco San Raffaele (Rome); ⁴IBPM CNR (Rome)

PMS.2 · P51    

Expression, purification and preliminary characterization of NS1 mutans proteins from highly patogenic avian influenza strains
R. Cancio Fernández¹, L. Bavagnoli¹, E. Crespam¹, W.G. Dundon², I. Capua³, G. Maga^1
1IGM/ CNR DNA Enzimology and Virology (Pavia); ²OIE, FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza (Newcastle, United Kingdom); ³Istituto Zooprofilattico Sperimentale delle Venezie (Venice)